The Calgary Metabolomics Research Facility (CMRF) is a state-of-the-art mass spectrometry and computational laboratory built specifically for unravelling the complex metabolic interactions that occur during human infections. These projects are supported by three independent analytical platforms, extensive bioinformatic resources, and expert staff who assist in the training and use of the specialized infrastructure.
Our discovery platform was designed to accommodate challenging metabolite identification projects. The platform includes a Thermo-Fisher Scientific Q-Exactive HF (QE-HF) high-resolution mass spectrometer and a Thermo-Fisher Vanquish ultrahigh performance liquid chromatography (UHPLC) separation system.
Our generalized metabolic profiling platform is built around a dedicated high-resolution mass spectrometer, a Thermo Fisher Scientific Q-Exactive Basic (QE-B), coupled to a Vanquish Flex UHPLC configured for ion-pairing chromatography. This configuration is ideal for quantifying central carbon metabolites and tracks over 250 metabolites covering core metabolic pathways.
For targeted projects, we use a dedicated Thermo Fisher Quantum Access Max triple quadrupole mass spectrometer (QqQ) coupled to a Vanquish Flex UHPLC. This system is ideal for quantitative analyses and for confirming metabolite assignments.
Each of our high-resolution metabolomics platforms can generate >1 GB of data per hour. We have a suite of bioinformatics tools, including 80 TB of storage and dedicated processing sever, for translating this stream of data into biologically meaningful information about pathogenic organisms.